A digestibility study conducted by São Paulo State University scientists determined the nutritional value of various ingredients for dog foods. Eight adult dogs were housed in metabolic cages, and distributed into 8 × 8 Latin square experimental design, with eight diets and eight replicates per diet. The difference, or substitution method was used to estimate ingredient digestibility.
A reference diet was formulated based on nutritional requirements of dogs. For each test diet, 300 g/kg of the reference diet was replaced with the ingredient evaluated. All the diets were extruded.